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(2020) Identification of Persistent Sulfidogenic Bacteria in Shale Gas Produced Waters

Cliffe L, Nixon S, Daly R, Eden B, Taylor K, Boothman C, Wilkins M, Wrighton K & Lloyd J


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08f: Room 3, Tuesday 23rd June 05:51 - 05:54

Listed below are questions that have been submitted by the community that the author will try and cover in their presentation. To submit a question, ensure you are signed in to the website. Authors or session conveners approve questions before they are displayed here.

Submitted by Barbara Sherwood Lollar on Saturday 20th June 20:07
Thank you! I was gratified to see the pendulum has swung and we are once again seeing both culture-based techniques like MPNs integrated with non-culture-based approaches. Can you give us a sense of what additional insight you gained for this (and future) studies from using both lines of investigation for your systems? Thank you – question posed by Barbara Sherwood Lollar (barbara.sherwoodlollar@utoronto.ca)

Submitted by Jethro Sanz-Robinson on Sunday 21st June 20:52
Do you have any idea of the geochemical conditions (pH, cation content, mineral phases..) that stimulate growth of these sulfidogenic bacteria?

Submitted by Oliver Warr on Monday 22nd June 02:12
Hi, thank you for sharing a really interesting study. From the MPN study of Lollar et al. 2019 SRB's were also similarly identified in the deep subsurface. However, despite their presence (and the availability of molecular hydrogen and simple alkanes), the H2S was below detection. As a result, I was wondering under what conditions might you expect hydrogen sulfide to form in these environments?

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