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(2020) Environmental Implications of Metal Binding by the Novel Chalkophore Methanobactin

Semrau J, Gu B, DiSpirito A, Kang-Yun C, Liang X, Lu X & Dershwitz P

https://doi.org/10.46427/gold2020.2335

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13h: Room 4, Wednesday 24th June 22:09 - 22:12

Listed below are questions that have been submitted by the community that the author will try and cover in their presentation. To submit a question, ensure you are signed in to the website. Authors or session conveners approve questions before they are displayed here.

Submitted by Elizabeth Phillips on Tuesday 16th June 17:59
Has the effect of Ca2+ on demethylation been tested? If not, do you intend to test this?
No, we have not directly assayed the effect of calcium on demethylation (i.e we have not varied calcium concentrations and then measured effect). We could certainly do that. Interestingly, to the best of my knowledge, no one has examined what the effect of varying calcium concentrations might be on methanotrophic activity/gene expression. Something to consider...thanks! What we are doing at the moment to perhaps address this issue is purify the two forms of methanol dehydrogenase, and then with varying amounts of methanobactin, determine rates and extent of methylmercury degradation.

Submitted by Elizabeth Phillips on Tuesday 16th June 17:59
Has the effect of Ca2+ on demethylation been tested? If not, do you intend to test this?


Submitted by Susan Little on Wednesday 24th June 09:41
Hi Jeremy, very interesting work. I wondered if you have any plans to do any Cu isotope analyses along these lines? If not, but you might be interested, please do get in touch offline. All the best, Susan Little
Hi Susan, Thanks for the positive comments. In short, no, we've not done any copper isotope analyses. But it's an interesting thought. I will ping you off-line.

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